Physical mapping of complex genomes by cosmid multiplex analysis.
نویسندگان
چکیده
منابع مشابه
Physical Mapping of Bacterial Genomes
The use of physical maps for the description and comparison of bacterial genomes has dramatically increased in the last few years with the advent of powerful tools for the construction of physical maps of large DNA molecules. The availability of restriction enzymes that cut whole bacterial genomes only a few times together with the use of pulsed-field gel electrophoresis to resolve and size the...
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The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as the key strategy, reduces problems caused by the genome complexity and polypl...
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Res. 21:3405-3411. 4.Diatchenko, L., Y.F. Lau, A.P. Campbell, A. Chenchik, F. Moqadam, B. Huang, S. Lukyanov, K. Lukyanov et al. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93:6025-6030. 5.Gauchat, J.F., J.P. Aubry, G. Mazzei, P. Life, T. Jomotte, G. Elson and J.Y. Bonnefoy...
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We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived from two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 "M" (Lawrence Livermore National Laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been con...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.13.5030